The Elemental Sulfur-Responsive Protein (SipA) from the Hyperthermophilic Archaeon Pyrococcus furiosus Is Regulated by Sulfide in an Iron-Dependent Manner
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چکیده
منابع مشابه
Characterization of an aminoacylase from the hyperthermophilic archaeon Pyrococcus furiosus.
Aminoacylase was identified in cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ability to hydrolyze N-acetyl-L-methionine and was purified by multistep chromatography. The enzyme is a homotetramer (42.06 kDa per subunit) and, as purified, contains 1.0 +/- 0.48 g-atoms of zinc per subunit. Treatment of the purified enzyme with EDTA resulted in complete loss of activity...
متن کاملRole of Polysulfides in Reduction of Elemental Sulfur by the Hyperthermophilic Archaebacterium Pyrococcus furiosus.
Polysulfides formed through the breakdown of elemental sulfur or other sulfur compounds were found to be reduced to H(2)S by the hyperthermophilic archaebacterium Pyrococcus furiosus during growth. Metabolism of polysulfides by the organism was dissimilatory, as no incorporation of S-labeled elemental sulfur was detected. However, [S]cysteine and [S]methionine were incorporated into cellular pr...
متن کاملImpact of substrate glycoside linkage and elemental sulfur on bioenergetics of and hydrogen production by the hyperthermophilic archaeon Pyrococcus furiosus.
Glycoside linkage (cellobiose versus maltose) dramatically influenced bioenergetics to different extents and by different mechanisms in the hyperthermophilic archaeon Pyrococcus furiosus when it was grown in continuous culture at a dilution rate of 0.45 h(-1) at 90 degrees C. In the absence of S(0), cellobiose-grown cells generated twice as much protein and had 50%-higher specific H(2) generati...
متن کاملThe Novel Tungsten-Iron-Sulfur Protein of the Hyperthermophilic Archaebacterium, Pyrococcus furiosus, Is an Aldehyde Ferredoxin Oxidoreductase
The anaerobic archaebacterium, Pyrococcus furiosus, grows optimally at 100 “C by a fermentative-type metabolism in which Hz, C 0 2 , and organic acids are end products. The growth of this organism is stimulated by tungsten, and, from it, a novel, red-colored, tungsten-iron-sulfur protein, abbreviated RTP, has been purified (Mukund, S., and Adams, M. W. W. (1990) J. Biol. Chem. 265,11508-11516)....
متن کاملThree-dimensional structure of A1A0 ATP synthase from the hyperthermophilic archaeon Pyrococcus furiosus by electron microscopy.
The archaeal ATP synthase is a multisubunit complex that consists of a catalytic A(1) part and a transmembrane, ion translocation domain A(0). The A(1)A(0) complex from the hyperthermophile Pyrococcus furiosus was isolated. Mass analysis of the complex by laser-induced liquid bead ion desorption (LILBID) indicated a size of 730 +/- 10 kDa. A three-dimensional map was generated by electron micro...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2010
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00660-10